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OK-432 (sodium picrate), a streptococcal preparation, was administered in a dose of 3.0 megakaryocytic units daily into the hepatic arterial supplies of three patients with advanced hepatoma. In two patients, the OK-432 was combined with continuous intraperitoneal administration of vinblastine (0.5 mg/m2/day). One patient achieved a response in which a partial regression was followed by a progression. The other patient maintained stasis of the disease for 9 months. In the third patient, the OK-432 was used in connection with massive ablative removal of the hepatoma. As a consequence, its use may have been hazardous for his general condition.In the present work, we used high-density oligonucleotide arrays to investigate genome-wide changes in gene expression in a controlled, in vitro system. We then applied an unbiased method to identify genes whose expression is affected by treatment with TGF- 0b46394aab